MuSIC Project
Download MuSIC Data
MuSIC Data
- This page contains links for all of the data in each MuSIC map, including the released MuSIC maps and the underlying raw data used to create them. This page will be updated as new MuSIC maps are released.
U2OS MuSIC Map v1.0
- The U2OS MuSIC map
- The U2OS MuSIC map contains 5,147 proteins organized into 275 subsystems. This global map of subcellular structure was generated by integrating immunofluorescent images with biophysical interactions derived from AP-MS.
- U2OS MuSIC map subsystems
- The 275 subsystems in the U2OS MuSIC map in tabular format. Each row represents a subsystem in the map with information on enrichment analyses, subsystem genes, and links to subnetworks. The second tab describes the information in each column.
- U2OS immunofluorescent images
- There are 20,660 immunofluorescent images for 10,348 proteins in the U2OS cell line. This file contains a link to every RGB image used in the U2OS MuSIC map and the corresponding gene name and antibody.
- U2OS biophysical interactions
- The U2OS biophysical interaction network contains 36,842 edges between 7,543 unique proteins. This interaction network was derived from affinity purification-mass spectrometry experiments. MS raw files are available via MassIVE with the identifier MSV000097168.
- U2OS size exclusion chromatography data
- There are three technical replicates of SEC-MS data. These files contain the protein quantification results for each replicate. MS raw files are available via Proteome Xchange with the identifier PXD052362.
- 3D Structural models
- AlphaFold-Multimer 3D structural models for protein pairs in U2OS cell map assemblies.
HEK293 MuSIC Map v1.0
- The HEK293 MuSIC map
- The HEK293 MuSIC map contains 661 proteins organized into 69 subsystems. This global map of subcellular structure was generated by integrating immunofluorescent images with biophysical interactions derived from AP-MS.
- HEK293 MuSIC map subsystems.
- The 69 subsystems in the U2OS MuSIC map in tabular format.
- HEK293 immunofluorescent images
- There are 2,357 immunofluorescent images for 1,078 proteins in the HEK293 cell line. This file contains a link to every RGB image used in the HEK293 MuSIC map and the corresponding gene name and antibody.
- HEK293 biophysical interactions
- The HEK293 biophysical interaction network contains 56,553 edges between 10,961 unique proteins. This interaction network was derived from affinity purification-mass spectrometry experiments described in Huttlin et al.